Jose P. Jacob - Integrated Sciences

Jose P. Jacob - Integrated Sciences

Location: 
Seminar Room 2
Start Time: 
Mon, 26/08/2019 - 2:30pm
End Time: 
Mon, 26/08/2019 - 3:30pm

​Tapestri : Single Cell Targeted Solutions for High-Impact Applications

​Special seminar hosted by Dr Daniela Zalchenstein

An average read-out from conventional bulk sequencing misses the underlying genetic diversity across cell populations. The Tapestri Platform was developed to enable the accelerated and accessible detection of genomic variability within and across cell populations. The platform delivers targeted solutions for high-impact application areas, including blood cancers, solid tumors, and genome editing validation. This novel approach to single-cell DNA analysis paired with targeted gene panels offers a powerful strategy for detecting rare subclones, resolving mutational co-occurrence patterns, zygosity and reconstructing phylogenetic lineages. In addition, proof-of-concept studies demonstrate that linking single-cell genotype (DNA) with phenotype information (protein) has the power to quantitatively and unambiguously gain further insights into cancer biology and disease progression.
 
The Tapestri Platform is a complete solution for high-throughput single-cell targeted DNA analysis, delivering high-quality variant calling of SNVs and indels. Leveraging droplet microfluidics and a novel two-step workflow, high-multiplex PCR, cell barcoding and targeted amplification is performed on thousands of individual cells simultaneously. Single-cell libraries are prepared using optimized Tapestri kits and then sequenced on an any Illumina platforms. Data is then analyzed and visualized using the intuitive Tapestri Pipeline and Tapestri Insights Software.