Technology

The Screening Laboratory supports three fully automated liquid handling systems and various types of plate readers, enabling high capacity assays to be formatted using a wide range of technologies. The range of assay technologies supported for formatting molecular screens includes

• AlphaScreen™

• Time resolved fluorescence

• Fluorescence polarization

• Steady-state fluorescence

• Photometry

• Chemiluminescence

• ELISA

• Scintillation proximity

• Filtration-based separations

• Surface plasmon resonance (SPR) using the Biacore 4000

For cell-based screens, supported assays include:

• Reporter genes (luciferase, green/yellow fluorescent proteins, alkaline phosphatase, beta-galactosidase)

• BRET™ (protein-protein interactions)

• Cell-surface antigen expression

• Cytokine expression

• Cell proliferation and cytotoxicity

Libraries

• The WECC library is composed of approximately 114,000 “lead like” small chemical entities. This library is our screening starting point to discover new hit compounds.

• The epigenetic library is composed of 95 compounds described to interact with epigenetic targets

• The kinase library is composed of 276 compounds targeting kinases

• Targeted agents library

• The known drug library is composed of 3,700 developed drugs that are on the market or failed during clinical trial

• The malaria box library is composed of 400 compounds that have shown activity against the asexual blood stage of P. falciparum.