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- Associate Professor Kelly Rogers
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- A multi-pronged approach to targeting myeloproliferative neoplasms
- A new paradigm of machine learning-based structural variant detection
- A whole lot of junk or a treasure trove of discovery?
- Advanced imaging interrogation of pathogen induced NETosis
- Analysing the metabolic interactions in brain cancer
- Atopic dermatitis causes and treatments
- Boosting the efficacy of immunotherapy in lung cancer
- Building a cell history recorder using synthetic biology for longitudinal patient monitoring
- Characterisation of malaria parasite proteins exported into infected liver cells
- Deciphering the heterogeneity of the tissue microenvironment by multiplexed 3D imaging
- Defining the mechanisms of thymic involution and regeneration
- Delineating the molecular and cellular origins of liver cancer to identify therapeutic targets
- Developing computational methods for spatial transcriptomics data
- Developing drugs to block malaria transmission
- Developing models for prevention of hereditary ovarian cancer
- Developing statistical frameworks for analysing next generation sequencing data
- Development and mechanism of action of novel antimalarials
- Development of novel RNA sequencing protocols for gene expression analysis
- Discoveries in red blood cell production and function
- Discovering epigenetic silencing mechanisms in female stem cells
- Discovery and targeting of novel regulators of transcription
- Dissecting host cell invasion by the diarrhoeal pathogen Cryptosporidium
- Dissecting mechanisms of cytokine signalling
- Doublecortin-like kinases, drug targets in cancer and neurological disorders
- Epigenetic biomarkers of tuberculosis infection
- Epigenetics – genome wide multiplexed single-cell CUT&Tag assay development
- Exploiting cell death pathways in regulatory T cells for cancer immunotherapy
- Exploiting the cell death pathway to fight Schistosomiasis
- Finding treatments for chromatin disorders of intellectual disability
- Functional epigenomics in human B cells
- How do nutrition interventions and interruption of malaria infection influence development of immunity in sub-Saharan African children?
- Human lung protective immunity to tuberculosis
- Improving therapy in glioblastoma multiforme by activating complimentary programmed cell death pathways
- Innovating novel diagnostic tools for infectious disease control
- Integrative analysis of single cell RNAseq and ATAC-seq data
- Interaction with Toxoplasma parasites and the brain
- Interactions between tumour cells and their microenvironment in non-small cell lung cancer
- Investigation of a novel cell death protein
- Malaria: going bananas for sex
- Mapping spatial variation in gene and transcript expression across tissues
- Mechanisms of Wnt secretion and transport
- Multi-modal computational investigation of single-cell communication in metastatic cancer
- Nanoparticle delivery of antibody mRNA into cells to treat liver diseases
- Naturally acquired immune response to malaria parasites
- Organoid-based discovery of new drug combinations for bowel cancer
- Organoid-based precision medicine approaches for oral cancer
- Removal of tissue contaminations from RNA-seq data
- Reversing antimalarial resistance in human malaria parasites
- Role of glycosylation in malaria parasite infection of liver cells, red blood cells and mosquitoes
- Screening for novel genetic causes of primary immunodeficiency
- Single-cell ATAC CRISPR screening – Illuminate chromatin accessibility changes in genome wide CRISPR screens
- Spatial single-cell CRISPR screening – All in one screen: Where? Who? What?
- Statistical analysis of single-cell multi-omics data
- Structural and functional analysis of epigenetic multi-protein complexes in genome regulation
- Structural basing for Wnt acylation
- Structure, dynamics and impact of extra-chromosomal DNA in cancer
- Targeted deletion of disease-causing T cells
- Targeting cell death pathways in tissue Tregs to treat inflammatory diseases
- The cellular and molecular calculation of life and death in lymphocyte regulation
- The role of hypoxia in cell death and inflammation
- The role of ribosylation in co-ordinating cell death and inflammation
- Understanding Plasmodium falciparum invasion of red blood cells
- Understanding cellular-cross talk within a tumour microenvironment
- Understanding the genetics of neutrophil maturation
- Understanding the roles of E3 ubiquitin ligases in health and disease
- Unveiling the heterogeneity of small cell lung cancer
- Using combination immunotherapy to tackle heterogeneous brain tumours
- Using intravital microscopy for immunotherapy against brain tumours
- Using nanobodies to understand malaria invasion and transmission
- Using structural biology to understand programmed cell death
- Validation and application of serological markers of previous exposure to malaria
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Kelly Rogers - Image processing and analysis
Researcher:
Software packages available
Imaris x64 v7.4.2:
Visualisation and analysis software ideal for 3D and 4D datasets. Only available on “Jupiter” workstation in Image Analysis room (C4.18).
Metamorph v7.7.7.0:
Analysis software for 2D microscopy data. Including options for advanced cell scoring and filtering, Integrated Morphometry Analysis and Nuclei Counting modules. Available on “Neptune” workstation.
Volocity
3D/4D data visualisation and analysis package. Full version available on all Image Analysis workstations, and 6 seat site licence (see “software for your computer” section below. Demo version freely available.)
Matlab R2012
Mathematical programming suite available for specific numerical analysis of image data. Speak with Image Laboratory staff for advice or access.
Living Image 3.
For analysis of IVIS Spectrum (whole animal imaging) data.
Attovision
Capture and analysis of data from BD Pathway.
Zen 201
Acquisition software for Zeiss confocals.
Axiovision 64bit
Deconvolution of widefield data.
Analysis hardware
“Jupiter”
“Imaris” Machine.
HPZ800 workstation. 64bit Windows OS. 24GB RAM.
Intel(R) Xeon(R) X5675 Processors.
Asus EAH6970 Graphics Card.
“Neptune”
Also known as the “Metamorph” machine.
HPZ800 workstation. 64bit Windows OS. 24GB RAM.
Intel(R) Xeon(R) X5570 processors.
NVIDIA Quadro FX 4800 Graphics Card.
“Triton”
Laboratory laptop and “Matlab” machine.
(Not generally available to users, see staff for access).
Dell Precision M4600. 64bit Windows OS. 16GB RAM.
Intel(R) Core(TM) iz-2860QM processors.
NVIDIA Quadro 1000M Graphics Card.
Microscopy server
Network storage available for storing and moving large microscopy data sets.
Current capacity 10TB. For access see laboratory staff.
Software for your computer
ImageJ / Fiji
Open-source image editing and analysis software. Many plugins and options available. We suggest downloading Fiji preferentially (Fiji Is Just ImageJ) as it has useful prepackaged plugins and a few other minor advantages. Can open almost any image file format, including raw formats direct from microscope capture software.
Photoshop / Gim
Image manipulation software (Gimp is free and open-source equivalent of photoshop). Beware – image manipulation can make your images look nicer, but it may destroy any scientific merit they have. Speak with laboratory staff if you’re unsure what you may, and may not, do to your data.
Volocity
Demo is fully featured and freely available, but will have watermarks across all images and will not allow printing. Network licence is available to allow you to use full version from your desktop, speak with staff for access instructions.