Deciphering the heterogeneity of the tissue microenvironment by multiplexed 3D imaging

Deciphering the heterogeneity of the tissue microenvironment by multiplexed 3D imaging

Project details

Cells are the fundamental building blocks of our body. Diverse types of cells with distinct gene expression profiles together orchestrate the functions of tissues or drive disease pathology. A number of technologies have emerged recently to measure cellular transcriptomic and proteomic information with subcellular resolution. However, these methods have only been successfully applied to thin tissue sections, which are not representative of tissues in the 3-dimensional context. 

This project aims to develop and refine a method to enable 3D multiplexed imaging of biomolecules in healthy and pathological tissues (i.e. lung, bone marrow) with subcellular precision. The resulting organ-scale multiomics analysis will advance our understanding of basic biology and the origin for tissue heterogeneity. Students will combine molecular techniques, tissue processing and advanced imaging.

About our research group

The Centre for Dynamic Imaging is a leading advanced microscopy facility for biomedical research. We are a multidisciplinary team of scientists who specialise in confocal, light sheet, lattice light sheet and super-resolution microscopy techniques and image analysis. We develop and apply state-of-the-art microscopy techniques to tackle challenging medical problems.

This project will involve collaborations with the antibody production and advanced genomics facility at WEHI.


Email supervisors



Photo of Dr Raymond Yip
Advanced Technology and Biology

Associate Professor Kelly Rogers

Associate Professor Kelly Rogers at a microscope
Associate Professor
Division Head; Head, Centre for Dynamic Imaging

Project Type:

Lattice light sheet microscope

Optical microscopy has become one of the most powerful tools in medical research.

WEHI's Centre for Dynamic Imaging is advancing our understanding of how diseases develop, spread and respond to treatment.