Next generation CRISPR screens using iPSC

Next generation CRISPR screens using iPSC

Project details

CRISPR approaches have revolutionised many areas of science, including our ability to screen the function of every human gene in a single experiment. However, 99 per cent of our genome does not encode a gene, and therefore we have developed a next generation CRISPR screen to uncover the function of these unstudied regions.

We intend to apply our new CRISPR screening methodology to induced pluripotent stem cells (iPSC). These are human cell lines that can be differentiated into a wide variety of important cell types, such as macrophages or neurons. These screens will identify the critical regions of our genome regulating macrophage and neuronal functions, and likely to contribute to autoinflammatory or neurodevelopmental diseases.

About our research group

The Masters laboratory is interested in how alterations in our genome causes autoinflammatory and neurological diseases. We start projects based on alterations in the genome which are present in patients, or that have functional effects in CRISPR screens, and then look to identify the mechanistic link to disease. This has resulted in numerous publications, and translated to increased rates of diagnosis and improved health outcomes for patients suffering from previously uncharacterised or untreatable diseases.


Dr Sophia Davidson profile photo
Inflammation division

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