Novel cell death and inflammatory modulators in lupus

Novel cell death and inflammatory modulators in lupus

Project details

We have identified abnormal Siglec receptor levels in lupus patient cells. However, it remains unclear as to how Siglec receptors might signal to limit lupus-associated inflammation, and whether they might impact the abnormal cell death signaling that has been associated with lupus. Therefore, we wish to examine the composition of the Siglec receptor complex, the inflammatory and cell death pathways Siglec signaling impacts and, ultimately, how these responses are altered in lupus patient cells.

This project will 

i) examine the levels of Siglec receptors in lupus patients, and

ii) use genetic engineering to generate cells that lack specific Siglec receptors, or overexpress them.

This will allow a detailed molecular and biochemical assessment of the impact of too much, or too little, Siglec receptor activity.

About our research group

Our laboratory studies the receptors and signaling networks that cause cell death and promote inflammatory cytokine production. These processes, often connected, are required to protect against microbial infection and repair damaged tissues, but can also be pathologically activated in autoimmune and inflammatory diseases, such as lupus.  

To achieve these aims, our laboratory makes use of both patient samples and gene knockout models lacking specific cell death or inflammatory signaling components. We combine molecular biology and biochemistry techniques, such as time-lapse microscopy, protein-complex purification, mass-spectrometry and genetic screening, with in vivo disease models, to interrogate the role of cell death and inflammatory regulators in health and disease. 

 

Researchers:

Dr James Vince

Dr James Vince in a laboratory
Dr
James
Vince
Laboratory Head

Dr Maryam Rashidi

Dr Maryam Rashidi at a laboratory bech
Dr
Maryam
Rashidi
Postdoctoral Fellow

Project Type: